In vitro Antioxidant and free radical scavenging activity of Macrotyloma uniflorum dal from Kumauni region

Authors

  • Renu Singh Department of Biotechnology, Bhimtal Campus, Kumaun University
  • Manoj Kumar Singh Department of Biotechnology, Bhimtal Campus, Kumaun University
  • Lovy Raj Chandra Department of Biotechnology, Bhimtal Campus, Kumaun University
  • Deepa Bhat Department of Biotechnology, Bhimtal Campus, Kumaun University
  • Manmeet Singh Arora Department of Biotechnology, Bhimtal Campus, Kumaun University
  • Tapan Nailwal Department of Biotechnology, Bhimtal Campus, Kumaun University
  • Veena Pande Department of Biotechnology, Bhimtal Campus, Kumaun University

DOI:

https://doi.org/10.59415/ijfas.v1i1.19

Keywords:

Dolichos biflorus ; antioxidants; Phenol; Flavonoid; DPPH.

Abstract

The present study was carried out to evaluate the in vitro antioxidant activities of Methanol
extract of Dolichos biflorus dal ((DME)) commonly edible food from central Himalayans. Methods: This was
achieved by screening of the plant extracts at varying concentrations (20-200μg/ml), using 2,2-diphenyl-1-picryl-
hydrazyl (DPPH) radical scavenging activity, reducing power assay and hydrogen peroxide radical scavenging activity.
Results: Total phenol and flavonoid contents (92.10 ± 8.11 mg/ml GAE per 100 mg plant extract and 139.5 ± 55.09
mg/ml QE equivalent per 100 mg plant extract) were found respectively. Scavenging effect of DME was 4 times greater
than that of the synthetic antioxidant ascorbic acid. Conclusion: Result also suggests a close relations in between total
phenolic content and antioxidant activity, reducing power and radical scavenging effect on DPPH radicals, which proves
Dolichos biflorus has a potential source of useful natural antioxidants.

Author Biographies

  • Renu Singh, Department of Biotechnology, Bhimtal Campus, Kumaun University

    Department of Biotechnology, Bhimtal Campus, Kumaun University, Nainital-263136, Uttarakhand, India.

  • Manoj Kumar Singh, Department of Biotechnology, Bhimtal Campus, Kumaun University

    Department of Biotechnology, Bhimtal Campus, Kumaun University, Nainital-263136, Uttarakhand, India.

  • Lovy Raj Chandra, Department of Biotechnology, Bhimtal Campus, Kumaun University

    Department of Biotechnology, Bhimtal Campus, Kumaun University, Nainital-263136, Uttarakhand, India.

  • Veena Pande, Department of Biotechnology, Bhimtal Campus, Kumaun University

    *Corresponding author
    Full Address :
    Department of Biotechnology, Bhimtal Campus, Kumaun University,
    Nainital.
    Phone no. +911594 2248042
    E-mail: veena_kumaun@yahoo.com

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Published

2012-03-30

Issue

Vol. 1 No. 1 (2012): International Journal of Fundamental and Applied Sciences

Section

Articles

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How to Cite

In vitro Antioxidant and free radical scavenging activity of Macrotyloma uniflorum dal from Kumauni region. (2012). International Journal of Fundamental and Applied Sciences (IJFAS), 1(1), 6-9. https://doi.org/10.59415/ijfas.v1i1.19

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