Neutral α-mannosidase from fungus Aspergillus fischeri (NCIM 508) belongs to Class IIC subfamily
DOI:
https://doi.org/10.59415/ijfas.v1i4.34Keywords:
α-mannosidase gene, Aspergillus fischeri, Cloning, Sequencing, gDNAAbstract
BACKGROUD & OBJECTIVE: The α-mannosidase gene from Aspergillus fischeri was isolated by DNA and RNA
method. METHODOLOGY: Cloning of gene, isolation of recombinant plasmid and sequencing of gDNA gene was
carried out and bioinformatic analysis was done for gene to get intron-exon information and for sequence homology.
RESULTS:The α-mannosidase gene from Aspergillus fischeri is 1990 base pair long and sequence analysis showed
presence of three introns and four exons in the gene. BLAST analysis revealed 100% similarity to the Neosartorya
fischeri (A. fischeri) and 94% with Aspergillus fumigatus class I α-mannosidase reported in NCBI Gene Data Bank.
CONCLUSION: The neutral α-mannosidase from Aspergillus fischeri could belong to the Class IIC subfamily.
Downloads
Published
Issue
Section
License
Copyright (c) 2022 International Journal of Fundamental and Applied Sciences (IJFAS)
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
License Statement
This work is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0).
Authors who publish with International Journal of Fundamental and Applied Sciences (IJFAS) retain copyright of their articles and grant the journal the right of first publication.
This license allows others to share, use, and build upon the work—commercially or non-commercially—as long as appropriate credit is given to the original authors and source, and any changes are indicated.
The journal encourages open access and supports the free exchange of knowledge while ensuring proper attribution of original work.
