In vitro antioxidant profiling of Bambusa tulda Roxb. aqueous methanolic leaf extract growing in the forests of Kokrajhar district, BTAD, Assam, India

Abstract

Background: From an age-old period bamboo has been an important ingredient of traditional Asian medicines in
general and Chinese medicine in particular. Today people are searching over the use of herbal medicine instead of the
synthetic drugs. Objective: The aim of this study was to evaluate phytochemical, biochemical constituent and in
vitro antioxidant activity of aqueous methanolic leaf extract of Bambusa tulda. Bambusa tulda (Poaceae) is widely
distributed in North Eastern parts of the Country. Methodology: Phytochemical constituents of leaves were extracted
by hot extraction method. Aqueous methanolic extract showed that the presence of phenolic compound, alkaloids,
saponnins, flavonoids, steroids, anthraquinones, glycosides. The total phenolic, flavonoid and flavonol content in the
aqueous methanolic extract were determined by calorimetric assay as well as their antioxidant activity through
various chemical assays like DPPH radicals, hydrogen peroxide scavenging and reducing power assay. Results: The
total phenolic, flavonoids and flavonol content in aqueous methanolic extract were 17.494±0.01 mg GAE/g,
176.35±0.03 mg QE /g and 96.2±0.01 mg QE /g respectively. Conclusion: This may be first report to provide
evidence that the crude aqueous metanolic extract of Bambusa tulda leaf is a potential source of natural antioxidant.